Journal: G3: Genes|Genomes|Genetics
Article Title: The human RAP1 and GFAPɛ proteins increase γ-secretase activity in a yeast model system
doi: 10.1093/g3journal/jkad057
Figure Lengend Snippet: RAP1 and GFAPɛ colocalize with subunits of the γ-secretase complex. SH-SY5Y cells were grown, fixed, and probed with antibodies as indicated in each panel. Representative images are shown that include images of each fluorophore individually with a larger, merged image. The box in the merged images indicates the region that is increased in size for the inset at the top right corner. Arrows in the inset indicate examples of colocalization. a) RAP1 and GFAPɛ colocalize. Cells were probed with DAPI, antibodies to RAP1 and antibodies to GFAPɛ. b) RAP1 colocalizes with the γ-secretase subunit PSENEN. Cells were probed with DAPI, antibodies to RAP1, and antibodies to PSENEN. c) RAP1 and GFAPɛ colocalize with the γ-secretase subunit PS1. DAPI stain was not used in this image. Cells were probed with antibodies to PS1, antibodies to RAP1 and antibodies to GFAPɛ.
Article Snippet: The samples were first incubated with primary antibodies to GFAPɛ (described above) or PSENEN (rabbit polyclonal anti-PEN2 antibodies, Invitrogen, PA5-20302) in blocking solution.
Techniques: Staining